EVALUATION OF ANTIEPILEPTIC ACTIVITY OF HELIOTROPIUM INDICUM IN PTZ INDUCED SEIZURE MODEL

Chippa Ramya1*, Rajashekar Perusomula2, Hima Bindhu Joolakanti3, Bhavishya4

1,4. Vishnu Institute of Pharmaceutical Education and Research, Vishnupur, Narsapur, Medak, Telangana, India, 2. HOD & Associate Professor, Department of Pharmacology, Vishnu Institute of Pharmaceutical Education and Research, Vishnupur, Narsapur, Medak, Telangana, India., 3. Associate Professor, Department of Pharmaceutical Chemistry,Vishnu Institute of Pharmaceutical Education and Research, Vishnupur, Narsapur, Medak, Telangana, India.

Corresponding Author: Vishnu Institute of Pharmaceutical Education and Research, Vishnupur, Narsapur, Medak, Telangana, India, Email: ramyach0111@gmail.com

Citation: Chippa Ramya et.al, (2026). Evaluation of Antiepileptic Activity of Heliotropium Indicum In Ptz Induced Seizure Model.Eco Science Journal.2026 3 (10).

Copyrights © 2026, Chippa Ramya, Rajashekar Perusomula, Hima Bindhu Joolakanti, Bhavishya.This article is licensed under the Creative Common Attribution-NonCommercial-4.0-international-License-(CCBY-NC).

ASTRACT: Epilepsy is a neurological disorder marked by recurrent seizures arising from abnormal neuronal excitability and disrupted excitatory-inhibitory balance, with oxidative stress contributing significantly to disease progression. This study evaluated the antiepileptic potential of hydro alcoholic extract of Heliotropium indicum using a Pentylenetetrazole (PTZ)-induced seizure model in Wistar albino rats. Phytochemical screening confirmed the presence of alkaloids, flavonoids, Phenolics, tannins, saponins, Terpenoids, steroids, and glycosides in the extract. Male Wistar rats (n=6/group) were divided into five groups and administered PTZ (80 mg/kg, i.p.) to induce seizures, followed by treatment with the extract (200 and 400 mg/kg, p.o.) or sodium valproate (200 mg/kg, p.o.) as the standard. PTZ significantly shortened seizure latency and prolonged Clonic and tonic seizure durations relative to controls. Extract treatment produced a dose-dependent anticonvulsant effect, delaying seizure onset and reducing seizure duration, with the 400 mg/kg dose showing effects comparable to the standard drug. Biochemical analysis revealed that PTZ elevated brain malondialdehyde (MDA) while depleting antioxidant enzymes (SOD, CAT) and glutathione (GSH); extract administration reversed these changes, restoring antioxidant status and lowering lipid peroxidation. These findings indicate that H. indicum, likely through its flavonoid and phenolic constituents, exerts anticonvulsant, antioxidant, and neuroprotective effects, potentially by enhancing inhibitory neurotransmission and mitigating oxidative neuronal damage. The extract represents a promising candidate warranting further investigation for epilepsy management.

Keywords: Heliotropium indicum, Anticonvulsant activity, Pentylenetetrazole (PTZ), Oxidative stress, Antioxidant activity, Neuroprotection.